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Effects of temperature shifts and oscillations on recombinant protein production expressed in Escherichia coli

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Abstract

Escherichia coli is widely used host for the intracellular expression of many proteins. However, in some cases also secretion of protein from periplasm was observed. Improvement of both intracellular and extracellular production of recombinant protein in E. coli is an attractive goal in order to reduce production cost and increase process efficiency and economics. Since heat shock proteins in E. coli were reported to be helpful for protein refolding and hindering aggregation, in this work different types of single and periodic heat shocks were tested on lab scale to enhance intracellular and extracellular protein production. A single heat shock prior to induction and different oscillatory temperature variations during the induction phase were executed. The results showed that these variations influence protein production negatively. In other words, 45 and 50 % reduction in extracellular protein production were observed for the single heat shock and oscillated temperature between 35 and 40 °C, respectively. However, the oscillatory temperature approach introduced in this study is recommended as a tool to quantitatively analyze the effects of inhomogeneous temperature on cell physiology and productivity in large-scale bioreactors.

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Acknowledgments

This research was conducted within the concept of the PhD thesis of Mr. Mohammadhadi Jazini and the authors fully acknowledge joint supervision of professor Roosta azad, the professor of Sharif University of Technology, Iran.

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Authors and Affiliations

  1. Chemical and Petroleum Engineering Department, Sharif University of Technology, Tehran, Iran

    Mohammadhadi Jazini

  2. Institute of Chemical Engineering, Research Division Biochemical Engineering, Vienna University of Technology, Gumpendorfer Strasse 1a, 1060, Vienna, Austria

    Mohammadhadi Jazini & Christoph Herwig

Authors
  1. Mohammadhadi Jazini
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  2. Christoph Herwig
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Correspondence to Christoph Herwig.

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Jazini, M., Herwig, C. Effects of temperature shifts and oscillations on recombinant protein production expressed in Escherichia coli . Bioprocess Biosyst Eng 36, 1571–1577 (2013). https://doi.org/10.1007/s00449-013-0927-1

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  • DOI: https://doi.org/10.1007/s00449-013-0927-1

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